Functional Bacterial Cellulose-Based MXene (Ti3C2Tx) Electronic-Skin Patch for Accelerated Healing and Monitoring
Künye
İLHAN, Saliha Nur, Bahar AKYÜZ YILMAZ & Fatih ÇİFTÇİ. "Functional Bacterial Cellulose-Based MXene (Ti3C2Tx) Electronic-Skin Patch for Accelerated Healing and Monitoring". BME Frontiers, 6 (2025): 1-17.Özet
Objective: This study aims to develop and characterize electroactive hydrogels based on reduced
bacterial cellulose (BC) and Ti3C2Tx-MXene for their potential application in wound healing and real-time
monitoring. Impact Statement: The integration of Ti3C2Tx-MXene into BC matrices represents a novel
approach to creating multifunctional hydrogels that combine biocompatibility, electrical conductivity,
and mechanical durability. These properties make the hydrogels promising candidates for advanced
wound care and real-time monitoring applications. Introduction: Wound healing requires materials that
support cell growth, promote tissue regeneration, and enable real-time monitoring. MXenes, a class of
2-dimensional materials, offer unique electrical and mechanical properties, making them suitable for
biomedical applications. This study explores the integration of Ti3C2Tx-MXene with BC, a biopolymer known
for its excellent biocompatibility and mechanical strength, to create electroactive composite hydrogel films
for advanced wound care. Methods: Ti3C2Tx-MXene was synthesized by etching Ti3AlC2 with hydrofluoric
acid and integrated into BC pellicles produced by Gluconacetobacter xylinum. The composite hydrogel
films underwent characterization through x-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS),
Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA) to determine
structural, chemical, and thermal properties. Mechanical testing assessed tensile and compressive
strengths. Biological assessments, including cell viability, hemolysis rate, and protein expression, evaluated
biocompatibility and regenerative potential. Results: XRD confirmed the crystallographic structure of
MXene and BC composite film. XPS and FTIR validated the successful incorporation of MXene into the
film matrix. Composite hydrogel films demonstrated a tensile strength of 3.5 MPa and a compressive
strength of 4.2 MPa. TGA showed stability up to 350 °C, and the electrical conductivity reached 9.14 ×
10−4 S/m, enabling real-time monitoring capabilities. Cell viability exceeded 95%, with a hemolysis rate
below 2%. Protein expression studies revealed the ability to promote skin regeneration through collagen I,
K10, K5, and filaggrin expression. Conclusion: The BC/MXene composite hydrogel films exhibit important
potential as electronic-skin patches for accelerating wound healing and enabling real-time monitoring.
Their unique combination of mechanical durability, electrical conductivity, and biocompatibility highlights
their promise for advanced wound care applications.