Green Synthesis of Silver Nanoparticles from Thymus Vulgaris and Sambucus Nigra Extracts in Poly (Vinyl Alcohol) Nanofiber Matrix: In Vitro Evaluation
Citation
TAVUKÇUOĞLU,Özlem, Nilüfer Evcimen DUYGULU, Aylin ALTINBAY & Fatih ÇİFTÇİ. "Green Synthesis of Silver Nanoparticles from Thymus Vulgaris and Sambucus Nigra Extracts in Poly (Vinyl Alcohol) Nanofiber Matrix: In Vitro Evaluation". Industrial Crops & Products, 222.3 (2024): 1-14.Abstract
In the current study, silver nanoparticles (AgNPs) green synthesized from Thymus vulgaris (Thyme) and Sambucus
nigra (black elderberry) (BE) extracts and added into poly (vinyl alcohol) (PVA) solution to achieve antibacterial,
biocompatible, and bioactive nanofibers through electrospinning technique. The absorbance peaks of AgNPs
were found at 450 nm in the ultraviolet–visible (UV-Vis) spectrum, and AgNPs were found in (111) d-spacing in
high-resolution transmission electron microscope (HRTEM). The average particle sizes were measured as 66.30
± 7.70 nm and 20.03 ± 4.80 nm for ThymeAgNPs and BEAgNPs, respectively. 1 and 2 wt% content of the AgNPs
were added into the PVA solution and electrospun at 9 kV voltage and 5 ml/hr flow rate. The fiber morphologies
were investigated with the help of scanning electron microscopy (SEM) and HRTEM, and a decrease in the fiber
diameter and the porosity of the fibers was obviously observed. Chemical bond analysis was done using Fourier
transform infrared (FTIR), and the thermal behavior of the fiber was examined with a differential scanning
calorimeter (DSC). X-ray diffraction (XRD) and HRTEM provide the presence of AgNPs in PVA nanofibers. According
to antibacterial activity investigations the inhibition growth effect against Escherichia coli (E. coli) of
PVA/ThymeAgNPs was 11.6 % higher than that of PVA/BEAgNPs. The cell cytotoxicity tests at different concentrations
with L929 cells revealed better performance in PVA/Thyme nanofibers.