An LED-Based Super Resolution GPU Implemented Structured Illumination Microscope

Abstract

Fluorescence imaging of sub-cellular structures with sizes below the di raction limit is vital in understanding cellular processes. Relying on exciting the sample with di erent illumination patterns and image processing for the elimination of background uorescence, Structured Illumination Microscopy (SIM) provides imaging capability beyond di raction limit using relatively simple optical setups. Here, we present a laser-free, DLP projector-based, and GPU-implemented SIM super resolution microscope. Sub-di ractive biological structures were imaged with a lateral resolution of 150 nm. The microscopy system is LED-based and entirely home-built which enables customizable operation at a low cost.