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Cellphone-Based Hand-Held Microplate Reader for Point-of-Care Testing of Enzyme-Linked Immunosorbent Assays

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Erişim

info:eu-repo/semantics/openAccess

Tarih

2015

Yazar

Berg, Brandon
Cortazar, Bingen
Tseng, Derek
Özkan, Haydar
Feng, Steve
Wei, Qingshan
Chan, Raymond Yan-Lok
Burbano, Jordi
Farooqui, Qamar
Lewinski, Michael
Di Carlo, Dino
Garner, Omai B.
Özcan, Aydoğan

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Künye

BERG, Brandon, Bingen CORTAZAR, Derek TSENG, Haydar ÖZKAN, Steve FENG, Qingshan WEİ, Raymond Yan-Lok CHAN, Jordi BURBANO, Qamar FAROOQUI, Michael LEWİNSKİ, Dino Dİ CARLO, Omai B. GARNER, & Aydoğan ÖZCAN. "Cellphone-Based Hand-Held Microplate Reader for Point-of-Care Testing of Enzyme-Linked Immunosorbent Assays." Acs Nano, 9.8 (2015): 7857-7866.

Özet

Standard microplate based enzyme-linked immunosorbent assays (ELISA) are widely utilized for various nanomedicine, molecular sensing, and disease screening applications, and this multiwell plate batched analysis dramatically reduces diagnosis costs per patient compared to nonbatched or nonstandard tests. However, their use in resource-limited and field-settings is inhibited by the necessity for relatively large and expensive readout instruments. To mitigate this problem, we created a hand-held and cost-effective cellphone-based colorimetric microplate reader, which uses a 3D-printed optomechanical attachment to hold and illuminate a 96-well plate using a light-emitting-diode (LED) array. This LED light is transmitted through each well, and is then collected via 96 individual optical fibers. Captured images of this fiber-bundle are transmitted to our servers through a custom-designed app for processing using a machine learning algorithm, yielding diagnostic results, which are delivered to the user within ∼1 min per 96-well plate, and are visualized using the same app. We successfully tested this mobile platform in a clinical microbiology laboratory using FDA-approved mumps IgG, measles IgG, and herpes simplex virus IgG (HSV-1 and HSV-2) ELISA tests using a total of 567 and 571 patient samples for training and blind testing, respectively, and achieved an accuracy of 99.6%, 98.6%, 99.4%, and 99.4% for mumps, measles, HSV-1, and HSV-2 tests, respectively. This cost-effective and hand-held platform could assist health-care professionals to perform high-throughput disease screening or tracking of vaccination campaigns at the point-of-care, even in resource-poor and field-settings. Also, its intrinsic wireless connectivity can serve epidemiological studies, generating spatiotemporal maps of disease prevalence and immunity.

Bağlantı

https://pubs.acs.org/doi/10.1021/acsnano.5b03203
https://hdl.handle.net/11352/3223

Koleksiyonlar

  • Biyomedikal Mühendisliği Bölümü [106]
  • Scopus İndeksli Yayınlar / Scopus Indexed Publications [630]
  • WOS İndeksli Yayınlar / WOS Indexed Publications [568]



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